Obgyn · Year 3 · from Obgyn

Case 1: Abnormal Pap Smear with HSIL

Patient Demographics

  • Age: 32 years
  • Sex: Female
  • Occupation: Dental hygienist

Chief Complaint

"I got a call that my Pap smear was abnormal and I need to come in for more testing."

History of Present Illness

The patient is a 32-year-old G2P2 who presents for colposcopy after an abnormal Pap smear. Her routine cervical cancer screening performed 2 weeks ago revealed high-grade squamous intraepithelial lesion (HSIL). She is understandably anxious and wants to know what this means and what to expect today.

Gynecologic History

  • Menarche: Age 12
  • Menstrual cycles: Regular, 28-day cycles, 5 days of flow
  • Last menstrual period: 10 days ago
  • Prior Pap smears: Normal at ages 25, 28, 30
  • HPV vaccination: Not vaccinated (not available during adolescence)
  • Contraception: Copper IUD placed after last pregnancy
  • STI history: Treated for chlamydia at age 22
  • Sexual history: 3 lifetime partners, currently monogamous

Obstetric History

  • G2P2002
  • Two uncomplicated vaginal deliveries at term

Pap Smear Result

  • Cytology: High-grade squamous intraepithelial lesion (HSIL)
  • HPV testing: Positive for high-risk HPV (type 16 detected)

Pre-Colposcopy Counseling

Explained to patient:

  • HSIL indicates precancerous changes that require further evaluation
  • Colposcopy involves magnified examination of the cervix
  • Biopsies may be taken, which causes mild cramping
  • This is NOT cancer, but needs treatment to prevent cancer

Colposcopy Procedure

Visualization:

  • Adequate colposcopy (transformation zone fully visible)
  • IUD strings visualized, not removed

After Acetic Acid (3-5%) Application:

  • Large area of dense acetowhite epithelium at 12-3 o'clock
  • Sharp, distinct margins
  • Punctation pattern visible (stippled appearance)
  • Mosaic pattern at 2 o'clock
  • No atypical vessels

Schiller's Iodine (Lugol's):

  • Non-staining area corresponding to acetowhite lesion
  • Confirms abnormal epithelium (lacks glycogen)

Biopsies Taken

  • Directed biopsies x 3 from most abnormal-appearing areas
  • Endocervical curettage (ECC) performed

Histopathology Results (1 week later)

Cervical Biopsies:

  • CIN 3 (carcinoma in situ) at 12 o'clock position
  • CIN 2 at 2 o'clock position
  • CIN 2 at 3 o'clock position

Endocervical Curettage:

  • Negative for dysplasia

Diagnosis

Cervical Intraepithelial Neoplasia 3 (CIN 3)

  • High-grade precancerous lesion
  • HPV 16 positive (highest-risk type)
  • No invasion on biopsy

Treatment Recommendation

Loop Electrosurgical Excision Procedure (LEEP)

Procedure Discussion:

  • Outpatient procedure under local anesthesia
  • Wire loop removes transformation zone
  • Provides tissue for histologic evaluation
  • Confirms margins and rules out invasion
  • High cure rate (>90%)

LEEP Procedure (performed 2 weeks later)

Findings:

  • Local anesthesia: Lidocaine with epinephrine
  • Cervix visualized with colposcope
  • Transformation zone excised with single pass
  • Specimen oriented and sent to pathology
  • Hemostasis with Monsel's solution
  • EBL <5 mL

LEEP Pathology:

  • CIN 3 present
  • Margins: Negative (clear ectocervical and endocervical margins)
  • No invasive carcinoma

Post-Treatment Surveillance

  • Counseled on importance of follow-up
  • HPV/Pap co-testing at 6 months
  • If negative, annual co-testing for 3 years
  • Then return to routine screening if all negative
  • Elevated cancer risk persists for 25 years; continued screening essential

Clinical Image

Image Description: Colposcopic image of the cervix after application of acetic acid demonstrating a large area of dense acetowhite epithelium with sharp borders and punctation pattern, characteristic of high-grade cervical intraepithelial neoplasia.

Attribution: Image from Wikimedia Commons. Educational colposcopy image. Licensed under CC BY-SA 4.0.


All cases for this lecture as Markdown